Tag Archives: Urticaceae

Exciting images of nettle flowers

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Partially dissected female flower of the subtropical shrub, Pouzolzia zeylanica showing the ovary exposed (left) and the elongated style and stigma (right) protected by tusk-like hairs. Image by Jia Dong, RBG Edinburgh

Working with Royal Botanic Gardens, Edinburgh student Jia Dong and plant anatomist Louis Ronse De Craene has resulted in some exciting and thought-provoking images of nettle flowers. The aim of our collaboration is to understand how nettle flowers develop and in the process work out what parts they have in common and which they don’t. The samples used were from living collections at Edinburgh and RBG Kew, together with my own collections in alcohol made over several years. The results are some beautiful and very informative scanning electron micrographs which show that the part of the female flower which recieves pollen (stigma) and conducts it to the egg (style) is characterised by two classes of hairs, one comprising defensive tusk-like hairs (above) and the other receptive tubular like hairs (above & below).

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Stigma hairs at the tip of the ovary of Pilea grandifolia, a succulent herb from Jamaica. Stigma hairs in the nettle family are characterised by their rounded obtuse tips and cylindrical shape. Image by Jia Dong, RBG Edinburgh.

Tubular hairs associated with the stigma are characteristic of all nettle flowers. They also appear very early in development. Combined this makes us think that they might have a role in pollination. Specifically in the reception of pollen. Being wind-pollinated, nettles don’t have a lot of control as to whose pollen reaches their female flowers and so there needs to be a way for them to control which pollen grains develop and fertilise the single egg. It seems likely that these hairs play a role and hopefully Jia will be able provide some more great images to test some hypotheses about this.

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Female flower of Cecropia sp., a tcommon ropical tree from the Americas. In addition to stigma hairs you can see an apparent fold in the ovary. Image by Jia Dong, RBG Edinburgh.

 

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Herbarium visit to La Paz (LPB)

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The sorting bench where incoming material is identified in the La Paz (LPB) herbarium

One important task for specialists in a particular plant group, in my case nettles, is to visit national or regional collections and not just rely on the collections of our own institutes, no matter how good they are. As part of a conference and field trip I have just spent a couple of days in the La Paz herbarium. My colleague Nicholas Hind will spend three weeks there identifying plants from the daisy family (Compositae) and running an identification course. There were 92 boxes of unidentified Compositae waiting for him when we arrived!This herbarium was founded by German botanist Stephan Beck in 1984 and currently houses over 400,000 herbarium specimens. The reason why such visits are important both for the specialist but also for the herbarium are that although there is an active inter-herbarium loan system for plants it relies on material being accessioned, mounted and identified. This can be a real challenge to achieve in a country where there are few funds and even fewer botanists. The visit of a specialist allows material that has not been mounted or accessioned, together with unidentified material, to be reliably (one hopes) identified and so used as reference for future identifications. Continue reading Herbarium visit to La Paz (LPB)

Ghost flowers in the nettle family

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A pickled Flower of Boehmeria zollingeriana, 1.2 mm in length viewed under a Zeiss Axioskop microscope. You can clearly see the two stigmas (ling filament like structures) and if you look carefully the two overlapping eggs within the ovary (dark egg-shaped structures)

Nettles are characterised, amongst other things, by having flowers with a single egg in their ovary and a single stigma, the structure which conducts the pollen to its target. Work by developmental biologists almost a century ago suggested that the ancestor of nettles probably had two eggs per ovary after discovering that at a very early stage of development nettle ovaries contain two eggs one of which disappears as the flower develops resulting in the single egged flower which characterises the family. It was therefore a great surprise when plant collections from Costa Rica examined in the 1990s were found to have flowers with two or three eggs and stigmas per ovary. These very unusual plants were described as a new species: Boehmeria burgeriana  by colleagues Melanie Wilmot-Dear and Ib Friis. Continue reading Ghost flowers in the nettle family

Soleirolia, a genus of small but perfectly formed nettles

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Close-up of a flowering stem of Soleirolia soleirolia showing the male (left) and female (right) flowers. The leaves of this tiny creeping herb are about 3 mm across

I first came across this tiny creeping herb in my garden where it had been planted as an ornamental. The bright green leaves, mostly less than 3 mm across form an attractive carpet. Until now I had never been able to spot its flowers despite having checked several times over the last few years. My guess is that this species has a relatively narrow flowering time in spring and the flowers are so tiny that they are only visible with a hand-lens. For several years the genus has intrigued me, not so much because of its small size and creeping habit but because of its distribution and evolutionary relationships.

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Engraving  of a female Soleirolia flower produced by the Anglo-French botanist Hugh Algernon Weddell in the 1850s. Weddell must have had access to microscopes of the very highest quality to produce such a drawing as the flowers that are about 1.5 mm in length

Firstly because Soleirolia consists of a single species that in the wild is only known from the Mediterranean island of Corsica. This is the only genus of nettle I know that is restricted to a single island or to the Mediterranean and I am very keen to try and found out why this could be (the history of the Mediterranean basin is quite a turbulent one). Secondly Soleirolia has traditionally been grouped with the  widespread Parietaria and intriguingly with Gesnouinia,  which also includes a single species but is restricted to the Canary Islands in the Atlantic Ocean. Whilst they look very different as plants their female flowers share many similarities of form.  It might be, therefore, that Soleirolia and Gesnouinia should be viewed  as Parietaria species that have diverged morphologically as a consequence of being isolated on islands, a common phenomenon in evolutionary biology. I am currently testing this possibility using DNA sequence data and could have a better idea in a couple of months.

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Endemic to Cosrica but now an ornamental and escaped weed throughout much of the temperate World Soleirolia soleirolia forms bright green carpets of tiny leaves

 

 

Seminar at the Royal Botanic Garden Edinburgh on the nettle family

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View of the tropical glass house at the Royal Botanic Garden, Edinburgh

Last week I was lucky enough to be invited to present a seminar on  the nettle family, the Urticaceae at the Royal Botanic Garden Edinburgh. I have been working on this group of plants for over fifteen years with various collaborators  and am finally ready to publish a revised classification of the family. I had a good audience who asked some insightful questions as well as making some good suggestions for future research. You can see a pdf of my slides by clicking on the following link: Edinburgh Urticaceae 19-6. Afterwards I had a chance to talk to a number of colleagues that I would like to start collaborating with in the future.

Solving the mystery of Myriocarpa flowers


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Composite image of the base of a female flower showing what we now believe are bracts at the base. Note the small stalked glands spaced evenly along each bract. You can see each cell in the flower thanks to the amazing imaging facilities at the Natural History Museum.

For over a hundred years the genus Myriocarpa in the nettle family which comprises ca 15 tree species in South and Central America has been impossible to place within the family. This is largely because of the very unusual shape of the part of the female flower that receives pollen, known as the stigma (see image below) and the fact that neither of the two great experts could agree over whether the petal-like structures at the base of the flower were petals associated with the flower, or bracts associated with the stalk. Whilst this might not seem like the stuff to keep a botanist awake at night it has become of interest again as using DNA data we have identified as sister to another small group of trees, Gyrotaenia, found in Cuba, Hispaniola, Jamaica and Central America that has a flower which consists of the petals fused to form a tube which is fused to ovary.

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The tip of the female Myriocarpa flower showing the very unusual forked stigma, the part of the flower which receives the pollen. You can see that it is covered in multicellular hairs, which are characteristic of the nettle family, and serve to capture pollen form the air and guide it to the stigma.

I was therefore very curious to see whether Vladimir Blagoderov, Manager of the Museum’s Sackler Imaging Suite could help me generate an image that would help us resolve the mystery. He could! The two images above are each composed of about 20 images which ‘slice’ through the sample which was of young flowers collected in alcohol in Belize over 10 years ago. The resolution was amazing, each cell being visible. In fact you could even make out the rough crystalline structure on the surface of the hairs! Both of these images also helped us to answer the question, revealing that this flower does indeed consist of a tube composed of fused petals that is subsequently fused to the ovary. This we could see in both images where the clearly visible ovary is enveloped by another distinct tissue, as in the case of Gyrotaenia. It was also confirmed by the petal-like bracts at the base of the flower having stalked glands, structures not known to occur on the ovaries of Urticaceae. So a morning’s work and an idea of evolutionary relationships enabled me to answer a question that had been frustrating an albeit very small group of botanists for over 100 years!

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Photograph of the string-like flower clusters of Myriocarpa longipes taken in Panama where it occurs as a small tree growing near rivers in tropical forest. Each flowers cluster consists of thousands of tiny flowers

 

 

Caves explored last month


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Yin Jia cave close to Gu Lin village in Yunnan. This is the 37th cave we have explored. At 1600 m above sea-level this is also one of highest elevations that we have collected in caves. Click to see a clip of the cave entrance

Thanks to funding from the Bentham Moxon Trust and the Guilin Botanical Garden, myself and colleagues explored five caves for plants in October of this year (2014). There are likely thousands of caves in the limestone karsts of south-east asia which contain plants. Whilst of great interest botanically and for conservation they are also beautiful in their own right and each cave is unique. I thought I would provide a portrait of each one to show how varied they are in their form, where plants grow in them and their size.

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Cave #40. We spotted this cave from the road and getting to it involved climbing onto an aquaduct that ran along the base of a large cliff and then jumping off into the cave. The elevation of this cave is 660 m above sea-level and it is located in the province of Guizhou.

The cave above was one of the few that we spotted from the road and then were able to get to. It is also one of the few that had little evidence of human disturbance, very few footprints and areas of pure white travertine that had fallen from the roof had not been walked on or collected. The main plant-bearing cavern of the cave was about 25 m deep and the roof 15 m high and it had a well developed flora, you can see plants from the african-violet family (Gesneriaceae) in the foreground and we collected five species of nettle here.

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This is the enormous Xiangshuidong Tian Keng cave in Guizhou, known to us as cave#38. Click to see a panorama from within the cave

Xiangshuidong Tian Keng cave in Guizhou was one of the largest caves that we have collected in, but also one of the most impacted by tourism and use by local communities. The main cavern is about 250 m deep with a roof between 45 and 30 m high and it is set within a huge cliff forming the side of a mountain. It also has a waterfall and river running across the back of it. It is in this cave that we found a very rare and unusual form of Elatostema oblongifolium that has its male flowers borne on specialised shoots but overall the plant diversity of the cave was quite low, presumably because of the large numbers of local tourists and associated trampling of much of the ground available for plants

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This is Cave #36, Yuen Ja cave close to Mahi Po Niochan village in Yunnan. The cave has two main chambers, each with their own entrance and which come together at their back where a new and unexplored chamber continues on. Each of the main chambers is 30 to 40 deep and the entrances 3 to 6 m high.

This was the first cave that we encountered on this field trip. We found it after first being taken to a hole in the ground as what must have been a mistranslation from Mandarin into the local dialect. The cave was relatively big and had a trail running inside. The main cavern shelves very steeply meaning that very little light penetrates into the cave. Despite this and the relatively high altitude, 1500 m, we collected seven species of nettle from here.

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Unfortunately I didn’t record the local name of this cave so it is known only as Cave 39. It is in the province of Guizhou at an elevation of 600 m. The main cavern was about 40m deep and  the entrance about 10m high and 30m wide. Click to see a panorama of the interior of the cave.

This cave should have been perfect as it shelved gently meaning that light penetrated quite deep, it also had plenty of places for plants to grow, such as boulders and rocks. We only collected four species of nettle here, probably because the cave was heavily impacted by farmers using it as a barn to keep their water buffalo in at night. Evidenced by lots of hoof prints and dung. This is a very common use of caves and the trampling of buffalo and their herders can have a significant impact on the plants in the caves (see below).

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Staminodes in nettles, an elegant use of ‘spare’ parts

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Fruit of Pilea japonica, ripe seeds are black and at their base you can see some white structures folded in on themselves. These are the modified stalks of remnants of male flower parts

Nettles have unisexual flowers, that is each flower functions only as a male or a female. Counter-intuitively though the flowers still retain the non-functional and often much-reduced organs of the non-functional sex. These are called pistillodes in the case of the rudimentary female organs in male flowers and staminodes in the case of the rudimentary male organs in female flowers.  In part of the nettle family the staminodes are put to good use: ejecting the seed from the fruit.

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Male flowers of Elatostema nanchuanense (left) showing the male flower parts, a pollen sack borne on a stalk and left their modification in the female flowers of the same species

This can be best understood by considering that nettles have male flowers which open explosively, pollen being released in tiny clouds (they are also very small). In fact one species, Pilea microphylla, is commonly known as the artillery plant for this reason. The mechanism for the explosive opening of the male flower is that the stalks (filaments) of the pollen sacks (anthers) are folded in on themselves in bud. As they develop these stalks fill with water until they are all pressing against each other within the flower bud and ready to burst. At a certain point the pressure becomes too great for the thin petals of the flower bud and they rip leading to the stalks being able to straighten explosively. This has happens incredibly quickly and although it has not been recorded for nettles, in the closely related mulberry where this also happens, the flower can open in 25 millionths of a second, moving petals to velocities in excess of half the speed of sound.

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Elatostema nanchuanense showing the fruiting flower head with staminodes, fruit and an ejected seed indicated by yellow arrows

In one group of nettles which includes seven genera this explosive ability of the pollen sacks has been harnessed to release the seed from the fruit. Below you can see a close-up of the fruit of Pilea japonica showing the staminodes flexed and ready to eject the seeds (dark coloured).

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Two weeks collecting in caves and gorges in South China

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Very steeply sided hills characteristic of limestone karst. This limestone was first exposed ca 240 million years ago and the karst produced by the action of rain over tens of millions of years

I have just returned from collecting nettles in South West China with my great friend and colleague Professor Wei Yi-Gang and researcher Fu Long-Fei from the Guilin Botanic Gardens. For several years we have been working on documenting the nettle diversity of the limestone karst of this region, focusing on the poorly known cave-associated floras. Karst is a form of limestone which has been weathered by rain for millions of years resulting in finely divided and sharp surfaces and very steeply sided hills, small mountains and gorges. The karst where we have been working form part of a formation which runs from Myanmar, northern Thailand and Vietnam and across into southern China and includes the famous ‘stone forests’ of Yunnan and Guangxi. I am interested in karst because it is where nettles are most diverse, both in terms of genera (species groupings) and species. At a single location it may be possible to encounter eight genera and over a dozen species!

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Yuan Jiá cave in north-eastern Yunnan close to the border with Vietnam at an elevation of 1500 m, we collected five species of nettle from this cave. Click on picture to see me introducing our work outside of the cave

Because the limestone is porous it has resulted in the formation of thousands of caves whose entrances have been colonized by plants from a small group of families: nettles (Urticaceae), african violet family (Gesneriaceae), Begonias (Begoniaceae), ivy family (Araliaceae), the coffee family (Rubiaceae) together with ferns and mosses. The most diverse of these are the nettles, one group of which, Elatostema has about 1/5 of the species from this region known only from caves. As well as having very low light levels, sometimes 1/10 of 1% daylight, the caves have constant humidity and low temperatures which contrast strongly with the cave exterior. The cool air of a cave can be felt up to 20 m from the entrance, often before the cave itself can be seen. We believe that the species associated with caves have likely come from the deep shade of the forests that once dominated this area but which have since been lost to agriculture. It also possible that some of the species are relicts of a previous, cooler climate during the last ice-age. This is something that we have begun to study.

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Elatostema binatum, one of over 15 species of Elatostema known only from caves in Guangxi, Yunnan and Ghuizou. This species is unusual in bearing male flowers on young shoots, female flowers have never been seen!

Caves represent a last refuge for several hundred species in SW China and now is a time of great change as caves come under threat from tourism, agriculture, urbanization and cement production. We want to help conserve these caves and the species that they include by first documenting their diversity and distribution across SW China. This information can be disseminated within China in the hope of raising awareness and protecting individual caves. It could also be used to identify a network of protected caves which include all of these species. Professor Wei would also like to develop protocols for cultivating the species outside of caves, something that is very difficult to achieve at the moment and which could represent a vital step for their conservation.

 

 

Cave-dwelling plants in SE Asia

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One of the many hundreds, if not thousands of caves located in the limestone karst of SW China. It is within such caves that we are discovering many new species of plants, very often from the nettle family.

Since 2007 I have been working with colleagues at the Chinese Academy of Sciences Guilin Institute of Botany on documenting the unusual cave flora of SW China and Guangxi. My interest stems from the fact that one of the most common groups of plants in these caves are two particular groups of nettles, members of the succulent herbaceous genera Elatostema and Pilea. It is also heavily influenced by the presence of a very knowledgeable and dedicated botanist at the Guilin Institute of Botany, Professor Wei Yi-Gang.

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Examples of some of the strange looking flower clusters in the nettle genus Elatostema, the most common group of nettles to be found growing in caves

More recently I have wanted to see whether it is possible to explain how, and when these plants occupied these ancient caves. Possible explanations are that they evolved in the caves, some of which are 15-25 million years old; alternatively that they represent plants which grew outside of the caves when the climate was different, during the last ice-age for example; lastly that they are relics of plants which grew in the forest understory outside of the caves prior to the arrival of agriculture in the area maybe 1,500 years ago. To try and answer these questions I have, together with a Masters student Alfred Gay, used DNA extracted from the leaves of the plants to look for patterns which may point to one of the three possible explanations above. Click here to see a slide show of the preliminary results.

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A carpet of nettles growing in the back of one of the caves that we surveyed in SouthWest China, taken with a tripod!

Another interesting line of research would be how these nettles survive in such low light levels. In some cases 1/50th of 1% daylight! For the moment though I am focussing on documenting their diversity and describing the new species we find but in the long-term I am hoping to find collaborators to explore these other areas of research.